The study investigated the effects of two magnesium formulations—sucrosomial magnesium and magnesium-buffered bisglycinate chelate—on human intestinal (Caco-2) and myometrial (PHM1-41) cells. The findings revealed that magnesium-buffered bisglycinate chelate exhibited superior intestinal absorption and more effectively promoted myometrial relaxation compared to sucrosomial magnesium, suggesting its potential efficacy in clinical applications.